Complementary sequence calculator8/28/2023 When the complementary strand is read backwards, the sequence is 5’-GGATCC-3’ which is identical to the first one, making it a palindromic sequence. 2 shows that this 9-base sequence exhibits perfect complementary to the 16S. This is the sequence where the restriction endonuclease, BamHI, binds to and cleaves at a specific cleavage site. The RBS calculator determined that the RBS206 (TTAATTAACCTCCTTCTTCGTT) with. An example of a palindromic sequence is 5’-GGATCC-3’, which has a complementary strand, 3’-CCTAGG-5’. ![]() single strands acts as a template for a new strand of complementary DNA. So if a sequence is palindromic, the nucleotide sequence of one strand would be the same as its reverse complementary strand. The nucleotide sequence of the human genome is now known to a reasonable degree. The pairing of nucleotides within the DNA double-helix is complementary which consist of Adenine (A) pairing with either Thymine (T) in DNA or Uracil (U) in RNA, while Cytosine (C) pairs with Guanine (G). Recognition sites of many restriction enzymes are palindromic. The sequence is the same when one strand is read left to right and the other strand is read right to left. Note having palindromic sequences along short the short stretches over which restriction enzymes function is not common.A DNA sequence whose 5'-to-3' sequence is identical on each DNA strand. Having short stretches of DNA that read the same on both strands of double-stranded DNA allow restriction enzymes to cut both strands in the same place.
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